1. Can you do an APIM self test? 2. Are you able to see the part number, calibration level, strategy, calibration, total distance?

I don’t think this is possible. Did the AsBuilt really change? Have you actually compared the hexadecimal values to confirm a change?

Yes, it's true.  I have seen in the Subaru world where people are using E85 because they claim they get better performance numbers on the dyno.   What nobody is saying, or realizing, is that you get may better performance but you are also burning the fuel faster due to the higher ethanol content fuel. So, is it really worth the cost over all buying cheaper E85 over the standard fuel? IMHO, absolutely not.   The only cars that should be using the E85 are those cars whose fuel systems are deliberately designed to run on E85.  FlexFuel systems are so designed so they go either way.   You must live out in the corn state area where corn is aplenty to use to generate the E85 fuel.

I don't have an explanation for what you're experiencing but can confirm that none of the changes I've made using Forscan have reversed themselves. 

So it's been a week since I finally was able to change 4 of my 2016 edge settings using Forscan. Simple things like disabling auto locks and seatbelt chimes. I get into the vehicle this morning and all of them are reset to the original values. I drive it on a daily basis. For no reason that I can understand this car continues to amaze (irritate) me. So my question is: is this normal? 

I didn't connect the dots the first time, and it is fairly rare to find Unleaded88, so I haven't filled up with it for a while. Once it happened again, I looked at my receipts and I had gotten Unleaded88 that last trip at some point. I will see if I can get the actual code set, but none of my code readers see a history/current/stored fault codes.   My winter gas is 15% ethanol and 87 octane, but this time of year it is only 10%. Since I would be burning 80-90% of it at once, and it won't sit in the tank, and 30 cents cheaper for better performance a negligible hit in mpg (I think it gets the same or better, as I have used it coming down from higher elevations, as well as going to higher elevations).   In May of this year a bill passed allowing year around distribution of E15, due to probable lack of gasoline (the one that everyone with a pre 2001 car freaked out about).

The owners manual (my 2019, your 2024) say specifically nothing over 15% ethanol, so Eco 88 should be fine.

How do you know for a fact that the sync3 you took out of the 2017 Ford Escape is directly compatible with your 2013 Edge?   Something is not talking to something else correctly, hence your problem. Consider researching how to reset the 2017 Ford Escape sync3 so that it can be reset / talk to work in your 2013 Ford Edge.

Yes, it typically will cost you more money but when you buy parts, make sure you are buying the real McCoy and  not some cheap knock-off.  Scammers selling cheap look-alike knock-offs are everywhere.  Companies, like Amazon for one, may buy from their Buyer but that doesn't mean what they bought was real.  Too many times a company's Buyer's instructions are to get the best price.  Best price on what is the real question.   Scammers are getting better at marking products to look just like the real thing.

So, each time you filled up with Eco88 fuel, you get this message.  What does that tell you?   Hmmm. . .    Do not buy Eco88 fuel.  Your system is telling you not to.